Sperm Freezing

Dr. Chandana Lakkireddi

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Sperm Freezing

Cryopreservation is a technique in which organelles, tissues, cells are stored at low temperatures to maintain their vitality. It is used to preserve sperms, eggs, and embryos for the process of in-vitro fertilization (IVF) or infertility treatments. The sperms, oocytes, embryos are usually thawed and cryopreserved to be used in the fertility treatment cycle.


IVF is a technology used for the treatment of infertility and gestational surrogacy.


Cryopreservation of Sperm

Sperm cryopreservation is a technique in which the spermatozoa of a healthy donor is extracted from the body and preserved at low temperatures for IVF and other infertility technology.


The successful storage period for human spermatozoa is 24 years. This technique helps in the management of male fertility before the treatment of any malignant disease that can have a negative or adverse effect on the quality of spermatozoa, such as- Vasectomy, chemotherapy, radiation therapy, and surgery.

Sperm Freezing

It is also a great technique to store donor sperm or ensure the recovery of a small number of spermatozoa in severe male factor infertility. For inquiries related to Sperm Cryopreservation consult today with the best IVF and Infertility treatment Clinic in Hyderabad, Dr. Chanadana Lakkireddi IVF Clinic.


Procedure

The sperm sample is collected in a dish and analyzed for volume, viscosity, and sperm motility.

Then, cryoprotectants are mixed into the sample. The best cryoprotectant used for sperm is Glycerol. They prevent the spermatozoa from any injury occurring during cryopreservation.


Following methods are used for cryopreservation of spermatozoa


Rapid freezing

It is a technique in which biological samples are quickly frozen so that large ice crystals cannot form and damage cells.
In this method, the sperms are directly immersed in the liquid nitrogen at -196°C within 8 to 10 minutes.
The drawback of this method is low reproducibility.


Slow freezing

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